Operators Manuals - VARIO XX28 - Issue 3
Warning - All external body and lid surfaces are hot during and
immediately after sterilisation. Lift or move the autoclave by
the top handle only.
Important - For correct operation of this autoclave it is
essential that the chamber be filled with water up to the height
of the studs before every use.
Foreword
Before using this Dixons Portable Autoclave please read this booklet carefully.
Please ensure that all persons likely to use this product are correctly
trained in its use in accordance with these instructions.
Your Dixons Portable Autoclave, operated in accordance with the manufacturers
instructions, will give years of trouble free service without the need
for any major servicing.
Every Dixons Portable Autoclave has been manufactured to the highest quality
standards by a company complying with BS EN ISO9000 and is fully tested
and ready for immediate use.
Dixons Portable Autoclaves are suitable for sterilisation of:
- Laboratory equipment (glass and metal)
- Culture media
- Pharmaceutical preparations in sealed containers
- Surgical and Veterinary instruments
- Dressings and drapes
For the sterilisation of dressings and drapes it is recommended that a
Dressing Drum or Wire Mesh Basket be used. These are available as a
part of the range of accessories.
All Dixons autoclaves are particularly suitable for destructive
sterilisation, as the operator is not restricted to a set cycle time.
This can be adjusted to suit the application.
1 - Installation Procedure
1.1 - On receiving the autoclave remove all the cardboard protective
covers. Unscrew the Wing Nuts and swing down the Drop Bolts. Twist the
lid anti-clockwise until the locking lugs disengage and remove the lid.
Remove all other packaging materials from inside the autoclave.
1.2 - Ensure that the following parts are included:
- Autoclave body and lid complete with all fittings as illustration
- Inner liner
- Pair of liner Carrying Handles
- White silicone gasket in lid
- Operators manual
- Test certificate
- Hexagon key for safety valve adjustment
- Pack of TST indicator strips
- Power lead with IEC connector
- VARIO automatic cycle control
- VARIO connecting lead
1.3 - Ensure that no damage has occurred to any of the fittings paying
special attention to the Pressure Gauge and Thermometer.
1.4 - The autoclave is now ready for use.
2 - Operation
Please read these operating instructions before using the autoclave
to avoid any chance of misuse.
The autoclave is factory set to run at 121ºC. This is the maximum
setting, which is suitable for most purposes. A lower setting can be used
if required.
Many materials can be autoclaved at 121ºC. These include most metals,
glass, silicone rubber and many plastics. If in doubt, consult the
manufacturer of the instrument or equipment concerned as the manufacturer
of the autoclave does not accept liability for damage of this nature.
2.1 - Remove the Inner Liner. Fill the outer chamber of the autoclave
with water up to the height of the studs.
2.2 - Articles to be sterilised should be placed into the Inner Liner, a
Wire Mesh Basket or a Bottle Rack. To sterilise porous loads (i.e. dressings and drapes)
it is recommended that a Dressing Drum be used. The container should not
be packed tightly, especially when dressings are being sterilised. It is
important that steam can circulate freely. Place the Inner Liner / Wire
Mesh Basket / Bottle Rack / Dressing Drum inside the autoclave.
2.3 - Check that the Lid Gasket is correctly seated in its groove
on the underside of the lid. Place the lid on the autoclave and rotate
clockwise until it hits the stops and the slots for the Drop Bolts line
up in the lid and body ring. The 6 Wing Nuts and Drop Bolts should now
be lifted up and lightly tightened in opposite pairs. This should be
done - one in each hand - until the slack is taken up. Now tighten up
in opposite pairs. Normal finger tightness is adequate.
2.4 - Close the Draw-off Cock.
2.5 - Plug the VARIO Connecting Lead into the Controller and into
the Connection Socket in the autoclave. Plug the power lead into the
autoclave. Plug the lead into the mains (the green light will show
connection to the mains). Turn on the red Switch (the red light will
show power on). The green light on the VARIO Controller will show power
to the controller.
2.6 - Select either one of the four preset cycles (A, B, C or D)
or the variable cycle. Section 5 deals with cycle parameters. The display
will show the parameters of the cycle chosen. If the variable cycle
is chosen, the parameters from the last time the variable cycle was
set will be shown. If you wish to change the parameters of the variable
cycle press the 'Set Minutes / Temp' button. This will cause the display
to flash. The setting can now be adjusted using the up and down buttons.
Section 5 deals with upper and lower limits of adjustment.
2.7 - Once the desired cycle is displayed press the green 'Start'
button to begin the cycle. The display will show 'Heating' and will
indicate the chamber temperature as it rises.
2.8 - When the water begins to boil, steam will start to emit from
the Self Seal Aircock. The Self Seal Aircock will freesteam until all the
air is displaced from the chamber and will then seal itself.
2.9 - The temperature will now continue to rise until it reaches
the preset level. The display on the controller will now show 'Sterilising'
and the minutes display will show a countdown of cycle time remaining.
2.10 - It is at this time that the sterilisation period begins.
Section 3 deals with sterilisation periods.
2.11 - When the minutes display reaches zero the sterilisation
period is complete. The display will now show 'Cooling'. At this
point the controller will emit a beeping sound five times. Depending
on the materials being sterilised, see section 2.12 or 2.13.
2.12 - Bottles or other sealed vessels containing liquid - do
not, under any circumstances, open the Draw-off Cock or release pressure
from the autoclave in any manner. To release pressure from the autoclave
at this point could cause the containers to explode. Allow the autoclave
to cool until the controller emits a beeping sound ten times
and the display shows 'Cycle Complete'. See section 2.14.
2.13 - Dressings, instruments and apparatus - (providing the
apparatus does not have sealed glass containers holding liquid). Place
a suitable receptacle beneath the Draw-off Cock and open the Draw-off
Cock to release water and steam under pressure from the autoclave. When
steam ceases to emit, close the Draw-off Cock. Allow the autoclave to
continue cooling until the controller emits a beeping sound ten times
and the display shows 'Cycle Complete'. If a quick cycle is required
see section 2.14. To make use of a full vacuum cycle allow the autoclave
to continue cooling until it is below 40ºC. At this point the temperature
display will be flashing '040'. See section 2.14.
2.14 - Check that the Pressure Gauge is reading zero. Unscrew
the 6 Wing Nuts in opposite pairs - one in each hand - 2 full turns. This
will allow the lid to rise to a safe position, where it is still held
captive, but has clearance enough to release any residual pressure still
in the Autoclave. Unscrew the 6 Wing Nuts fully in opposite pairs - one
in each hand - and swing down the Drop Bolts. Twist the lid anti-clockwise
until the Locking Lugs disengage. Remove the lid.
2.15 - Remove the sterilised contents.
3 - Sterilising Periods
The following times apply to a loosely packed load. Dressings in particular
should not be packed tightly as steam will not penetrate. Wire Mesh
Baskets or Dressing Drums are recommended for this application.
When large packs of dressings or large volumes of liquid are being
sterilised, it is advisable to allow a further 10 minutes sterilisation
time. This ensures adequate penetration of the materials by steam and heat.
Use these times as a general guide only - always use a sterilisation
test e.g. the TST integrator strips supplied.
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Instruments and Equipment
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15 minutes @ 121 deg C
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Dressings and Drapes
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30 minutes @ 121 deg C
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Liquid in Sealed Containers (0ml - 50ml)
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20 minutes @ 121 deg C
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Liquid in Sealed Containers (50ml - 100ml)
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20 minutes @ 121 deg C
|
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Liquid in Sealed Containers (100ml - 200ml)
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20 minutes @ 121 deg C
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Culture Media (up to 1 litre)
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15 minutes @ 121 deg C
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Notes on sterilisation of Culture Media:
1 - Always follow the manufacturer's instructions.
2 - Use volumes up to 1 litre.
3 - Use similar volumes in the same load.
4 - After cycle test for sterility.
5 - Check microbiological performance before use.
4 - Maintenance
UK users - this autoclave comes within the scope of 'The Pressure
Systems and Transportable Gas Containers Regulations 1989' and should
be inspected and maintained accordingly. General maintenance should
be carried out by a competent person only, as and when deemed necessary.
4.1 - Lid Gasket - Check the white silicone Lid Gasket for
distortion or wear and tear. The Lid Gasket is located in the groove
on the underside of the lid. Check that it has not become brittle.
Replace the Lid Gasket with a new one if necessary.
4.2 - Body Rim - Check the rim of the body where the Lid Gasket
seats for damage to the metal rim which would cause the lid seal
to leak. If the damage is only minor then the rim can be repaired by
filing and smoothing with emery cloth. Major damage should be referred
to the manufacturer or a qualified service engineer.
4.3 - Draw-off Cock - Check the Draw-off Cock for signs of leaking
around the seals and from the valve. Replace the seals or complete
Draw-off Cock with a new one if necessary.
4.4 - Cleaning - Use normal household detergent to clean the
interior of the autoclave.
4.5 - Safety Valve Adjustment and Maintenance - Please see our
Safety Valve Adjustment
document.
4.6 - Electrical Maintenance (by a qualified Electrician only)
- One element is internally fitted - 2.0kW - requiring a minimum 10A
fuse. Check that the terminals on the elements are tight and that
the elements are securely mounted in the base. Check the Switch and
light for damage. Replace if necessary. See that the IEC connector
fits firmly in the socket.
4.7 - Overheat Cutout - To avoid damage to the element, should
the autoclave boil dry, it is fitted with an automatic overheat
cutout. This is clamped to the base of the chamber. If the
device should cutout it must be manually reset. Before doing this switch
off the mains to the autoclave and remove the plug. Allow the autoclave
to cool down. To reset the cutout turn the autoclave upside down and
remove the perforated protection plate from the bottom. The cutout
will now be visible. It is a round black unit approximately 15mm in
diameter with a red button protruding from the middle. To reset the
cutout press this red button. You should hear an audible 'click' as the
cutout is reset. Refit the protection plate and turn
the autoclave the right way up. The autoclave will now work as before.
5 - Cycle Parameters
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Cycle A
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30 minutes @ 115 deg C
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Cycle B
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15 minutes @ 121 deg C
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Cycle C
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30 minutes @ 121 deg C
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Cycle D
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60 minutes @ 115 deg C
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Min Variable Temperature
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100 deg C
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Max Variable Temperature
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121 deg C
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Min Variable Time
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1 minute
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Max Variable Time
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99 minutes
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A - Draw-off Cock
B - Self Seal Aircock
C - Pressure Gauge
D - Safety Valve
E - Thermometer
F - Wing Nut (6)
G - Drop Bolt (6)
H - Switch
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K - Vario Automatic Cycle Controller
L - Vario Connection Socket
M - Vario Connecting Lead
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